The icon of life innovation.

Thursday, 22 February 2018

  CONTACT US

Tel : 02 692 9330,
        02 274 1291-5

  Work-Time

Monday-Friday time 08:00-17:00

  Address

Huai Khwang  Bangkok 10310

SYBR Green Real-Time PCR Master Mixes

     SYBR™ Green master mixes are designed for quantitative real-time PCR using a set of two PCR primers that flank the target region. The master mixes contain buffer, dNTPs, thermostable hot-start DNA polymerase, and of course, SYBR Green dye—everything needed for real-time PCR, except the sample and PCR primer pair.

Which SYBR Green master mix is right for you?

     SYBR Green Master Mix formulations have improved over the years, as researchers have demanded improved sensitivity, greater specificity, enhanced reproducibility, faster time-to-results, and protection from contamination. Although there are now many choices of SYBR Green master mixes available, below are the recommended master mixes, by application.

Applications Gene expression analysis
(Microbe detection,
ChIP DNA analysis)
Gene expression analysis
(Microbe detection,
ChIP DNA analysis)
Genotyping by high resolution melt (HRM) analysis
(Mutation scanning Methylation analysis)
 Recommended master mix  PowerUp™ SYBR™ Green Master Mix  Power SYBR™ Green Master Mix  MeltDoctor™ HRM Master Mix
 Performance  Best  Better  Best
 Hot start

 Dual-lock for exceptional specificity


 
 Cycling mode  Standard (~60 min) or
Fast (~30 min)
 Standard (~60 min)  Standard (~70 min)
 Carryover contamination control  Includes heat-labile UNG and a blend of dTTP/dUTP  Contains dUTP, which is compatible with AmpErase™ UNG (available separately)  Contains dUTP, which is compatible with AmpErase UNG (available separately)
 72-hour stability of reaction mixes prior to qPCR      
 Instrument compatibility  Compatible with all Applied Biosystems™ real-time PCR instruments, Bio-Rad iQ™5 and CFX™ (96 and 384), Roche LightCycler™ 480, and Stratagene™ Mx3005P™ systems  Compatible with all Applied Biosystems real-time PCR instruments, Bio-Rad iQ5, Roche LightCycler 480, and Stratagene Mx3005P systems  Compatible with Applied Biosystems real-time PCR instruments running High Resolution Melt Software
 Recommended primer concentration  300–800 nM  50–900 nM  50–900 nM

Looking for something else?

     See the entire range of SYBR Green master mixes available from Thermo Fisher Scientific below.

 

                 

 

 

 

Comments

 Applied Biosystems™ products          Wide instrument compatibility, and the best choice for Applied Biosystems instruments. All mixes include ROX™ dye.  
 PowerUp SYBR Green Master Mix (Recommended)                Our newest and best mix. Formulated for exceptional specificity, single-copy detection, and wide instrument compatibility.
 Power SYBR Green Master Mix (Best value)              Our most popular mix. A great value.
 SYBR Select Master Mix                Our brightest mix. May be too bright for some older instruments.
 Fast SYBR Green Master Mix          Our first mix formulated for fast cycling.
 SYBR Green Master Mix              The original Applied Biosystems™ SYBR Green master mix.
 MeltDoctor HRM Master Mix                Formulated specifically for high resolution melt (HRM) analysis.
 Thermo Scientific™ products          Mixes optimized for specific instruments from Thermo Fisher Scientific and other suppliers. Versions with separate ROX, low ROX, high ROX, and fluorescein available.
 Luminaris™ Color HiGreen qPCR Master Mix        Includes dye in mix and in sample buffer to aid pipetting.
Maxima™ SYBR Green qPCR Master Mix              A basic mix with great value, especially if you don’t want premixed ROX.
DyNAmo™ ColorFlash SYBR Green qPCR Kit        A fast cycling–compatible mix that includes dye in mix and in sample buffer to aid pipetting.
Absolute™ QPCR Mix, SYBR Green                A basic mix, and the only one available without dUTP.
Invitrogen™ products Mixes optimized for specific instruments from Thermo Fisher Scientific and other suppliers. Versions with separate ROX, low ROX, high ROX, and fluorescein available.
SYBR™ GreenER™ qPCR SuperMix            An updated, brighter version of Platinum™ SYBR Green supermix.
Express SYBR™ GreenER™ qPCR SuperMix          All the features of SYBR GreenER mix, but fast cycling–compatible. To be discontinued.
Platinum™ SYBR™ Green qPCR SuperMix-UDG            The original Invitrogen SYBR Green master mix.

1. • = master mix includes uracil N-glycosylase (UNG); •• = master mix includes a heat-labile form of UNG, which improves master mix performance compared to the standard UNG.

2. Master mix includes ROX, a passive reference dye; V = varies, different master mix versions are available with different amounts of ROX dye, or ROX included in a separate tube.