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Wednesday, 21 February 2024

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  • GENEPLUS CO.,LTD.

    Geneplus was founded in 2010. We do have a team of 30 employees with a strong background and knowledge in molecular biotechnology. With our product portfolio we cover the entire workflow in molecular biology.

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  • Ion Torrent l Applied Biosystems

  • Professional Instruments for Plant Science and Algae Biotechnology

    PlantScreen Systems - Complete Solutions for Automated Phenotyping

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SYBR Green Real-Time PCR Master Mixes

SYBR Green Real Time PCR Master Mixes

     SYBR™ Green master mixes are designed for quantitative real-time PCR using a set of two PCR primers that flank the target region. The master mixes contain buffer, dNTPs, thermostable hot-start DNA polymerase, and of course, SYBR Green dye—everything needed for real-time PCR, except the sample and PCR primer pair.

     SYBR Green Master Mix formulations have improved over the years, as researchers have demanded improved sensitivity, greater specificity, enhanced reproducibility, faster time-to-results, and protection from contamination. Although there are now many choices of SYBR Green master mixes available, below are the recommended master mixes, by application.

Applications Gene expression analysis
(Microbe detection,
ChIP DNA analysis)
Gene expression analysis
(Microbe detection,
ChIP DNA analysis)
Genotyping by high resolution melt (HRM) analysis
(Mutation scanning Methylation analysis)
 Recommended master mix  PowerUp™ SYBR™ Green Master Mix  Power SYBR™ Green Master Mix  MeltDoctor™ HRM Master Mix
 Performance  Best  Better  Best
 Hot start

 Dual-lock for exceptional specificity


 
 Cycling mode  Standard (~60 min) or
Fast (~30 min)
 Standard (~60 min)  Standard (~70 min)
 Carryover contamination control  Includes heat-labile UNG and a blend of dTTP/dUTP  Contains dUTP, which is compatible with AmpErase™ UNG (available separately)  Contains dUTP, which is compatible with AmpErase UNG (available separately)
 72-hour stability of reaction mixes prior to qPCR      
 Instrument compatibility  Compatible with all Applied Biosystems™ real-time PCR instruments, Bio-Rad iQ™5 and CFX™ (96 and 384), Roche LightCycler™ 480, and Stratagene™ Mx3005P™ systems  Compatible with all Applied Biosystems real-time PCR instruments, Bio-Rad iQ5, Roche LightCycler 480, and Stratagene Mx3005P systems  Compatible with Applied Biosystems real-time PCR instruments running High Resolution Melt Software
 Recommended primer concentration  300–800 nM  50–900 nM  50–900 nM

     See the entire range of SYBR Green master mixes available from Thermo Fisher Scientific below.

 

                 

 

 

 

Comments

 Applied Biosystems™ products          Wide instrument compatibility, and the best choice for Applied Biosystems instruments. All mixes include ROX™ dye.  
 PowerUp SYBR Green Master Mix (Recommended)                Our newest and best mix. Formulated for exceptional specificity, single-copy detection, and wide instrument compatibility.
 Power SYBR Green Master Mix (Best value)              Our most popular mix. A great value.
 SYBR Select Master Mix                Our brightest mix. May be too bright for some older instruments.
 Fast SYBR Green Master Mix          Our first mix formulated for fast cycling.
 SYBR Green Master Mix              The original Applied Biosystems™ SYBR Green master mix.
 MeltDoctor HRM Master Mix                Formulated specifically for high resolution melt (HRM) analysis.
 Thermo Scientific™ products          Mixes optimized for specific instruments from Thermo Fisher Scientific and other suppliers. Versions with separate ROX, low ROX, high ROX, and fluorescein available.
 Luminaris™ Color HiGreen qPCR Master Mix        Includes dye in mix and in sample buffer to aid pipetting.
Maxima™ SYBR Green qPCR Master Mix              A basic mix with great value, especially if you don’t want premixed ROX.
DyNAmo™ ColorFlash SYBR Green qPCR Kit        A fast cycling–compatible mix that includes dye in mix and in sample buffer to aid pipetting.
Absolute™ QPCR Mix, SYBR Green                A basic mix, and the only one available without dUTP.
Invitrogen™ products Mixes optimized for specific instruments from Thermo Fisher Scientific and other suppliers. Versions with separate ROX, low ROX, high ROX, and fluorescein available.
SYBR™ GreenER™ qPCR SuperMix            An updated, brighter version of Platinum™ SYBR Green supermix.
Express SYBR™ GreenER™ qPCR SuperMix          All the features of SYBR GreenER mix, but fast cycling–compatible. To be discontinued.
Platinum™ SYBR™ Green qPCR SuperMix-UDG            The original Invitrogen SYBR Green master mix.

1. • = master mix includes uracil N-glycosylase (UNG); •• = master mix includes a heat-labile form of UNG, which improves master mix performance compared to the standard UNG.

2. Master mix includes ROX, a passive reference dye; V = varies, different master mix versions are available with different amounts of ROX dye, or ROX included in a separate tube.


More Detail

Products & Solution

Professional distributor company in biotechnology

  1. Ion Torrent
  2. Applied Biosystems
  3. Rapid DNA
  4. Hamilton
  5. PSI
  6. qualitype
  7. Azure

The Ion GeneStudio S5 Series

The Ion GeneStudio S5 Series sequencers share the same foundational technology and fluidic architecture across all three instruments: the Ion GeneStudio S5 System, the Ion GeneStudio S5 Plus System, and this Ion GeneStudio S5 Prime System. The primary difference across the three instruments is on-sequencer analysis time.

     With >1,500 publications featuring Ion Torrent™ technology in the first 4 years since its introduction, now you too can drive your research forward with the latest innovation in benchtop next-generation sequencing.

 Genetic Analyzer for HID

Thermo Fisher Scientific offers you simple, complete, end-to-end workflows for forensic DNA analysis to help resolve difficult cases and provide investigative leads in a forensically responsible and relevant manner. We are committed to delivering unparalleled support and continued innovation to meet your needs today and tomorrow.  More Detail...

 

answers for every case

Rapid DNA Solutions—Because Every Minute Counts

Every minute that a crime remains unsolved is time for someone else to become a victim. Imagine what can happen when DNA testing takes only minutes, instead of days, weeks, or even months.  

Rapid DNA helps solve crimes today and prevent victims tomorrow
The compact, easy-to-use Applied Biosystems RapidHIT ID System generates forensic DNA profiles in virtually any setting in as little as 90 minutes—and no technical expertise is required. Safer communities are within reach.

     Hamilton Storage sample management systems are specifically designed to easily integrate with the Hamilton Robotics liquid handling workstations creating a comprehensive sample processing center that supports a broad range of applications. Additional devices such as decappers, readers, centrifuges, sealers and shakers can be integrated as well, providing a wide range of custom solutions for the customer. Our unique and simple approach offers our customers a complete solution that is specific to their needs.

     Our systems allow users to start with a simple, single format technique with the availability to adopt additional formats into our system's design. Users can combine standard modules to configure a system to fit specific automated sample management needs and budgetary guidelines without compromising future growth. 

     Whatever your current or future sample management needs are, Hamilton can offer a wide range of solutions to provide your lab with a complete, fully integrated automated sample management system. 

     PSI (Photon Systems Instruments) is a high-tech company located close to the industrial city of Brno in the south of Czech Republic. For more than 20 years PSI has been specializing in the design and manufacture of sophisticated, high-end instrumentation for research in biological sciences. The company is a world expert in manufacturing instrumentation for advanced imaging and non-imaging of optical signals in plants and algae, particularly in the field of chlorophyll fluorescence/reflectance techniques. The instrumentation developed over passed years is used for monitoring of wide range of physiologically relevant signals on the level of individual cells up to macroscopic level referring to few metres high crop plants.

Abetter LIMS is a laboratory information management system optimizing your lab routine from sample and master data management and structuring your daily work with planning functionalities for conduction of high-throughput analyses.

As a base for a successful accreditation the software supports quality assurance functions in compliance with ISO 17025 and GLP requirements. Thereby, complete sample tracking and audit trail are provided.

Recognizing the special demands on specialized labs, Abetter LIMS provides tailored expert modules for forensic or clinical areas.

 

 

 

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Ion Torrent Genexus System

  To bring next-generation sequencing (NGS) in-house, you need a solution that is truly easy to implement and easy to use, a solution that can help you surmount the current barriers and redefine in-house NGS. Imagine what you could achieve with a specimen-to-report NGS solution that delivers answers in a single day, cost-effectively runs even small batches, and can access all relevant biomarkers.

SARS-CoV-2 Research Solutions

From viral research and epidemiological studies to potential vaccine development.

As you work to understand SARS-CoV-2 (the coronavirus that causes the COVID-19 disease), to rapidly identify future treatment options and develop possible vaccine targets, don't worry about having the supplies and instrument support you need. Our comprehensive portfolio of research products is here to help advance your research.

BIOTECHNOLOGY VIDEO

infomations & events

NEWS & EVENTS

News infomations products and events geneplus

Human Identification Seminar 2014
HUMAN IDENTIFICATION
Reproductive Health Seminar
REPRODUCTIVE HEALTH SEMINAR
Ion world tour 2016
NEXT GENERATION SEQUENCING SEMINAR
THAILAND LAB INTERNATIONAL 2019
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About us

Geneplus Co.,Ltd. is a leading distributor company in the biotechnology and molecular diagnostic market in Thailand.

Company Profile

   With Automated DNA/RNA Extraction, Liquid Handling, PCR/QPCR, Saenger Sequencing, Next Generation Sequencing, DNA/RNA Fragment Analysis and our customers can choose their appropriate solutions.

  • We cooperate with dedicated high tech partners from several countries.
  • For all Technologies we do have specialized application support.
  • Furthermore we can offer LIMS solutions customized for Forensics, ClinicalApplications and food and animal health.
  • Since 2015 we do have a new technology so called “Plant Phenomics” which is a very fast developing market.

Geneplus Co.,Ltd. is a leading distributor company in the biotechnology and molecular diagnostic market in Thailand.

We are exclusive distributor for Applied Biosystems | Thermo Fisher Scientific which is a global leader in providing innovative instrument systems to accelerate academic and clinical research, drug discovery and development, pathogen detection, environmental analysis, and forensic DNA analysis.

Focus

If our customers are successful
we are successful, too!